Streamlined single plate protocols including all stimulation and cytokine quantification.
The HTRFâ cytokine kit line includes TNFa, IFNg, IL1b, IL6 and IL8 assays. These assays feature no separation steps, a high sensitivity and dynamics, and no use of isotopes. Procedures are performed on a single plate with simultaneous cell stimulation and cytokine detection. They can all be run under a similar experimental procedure streamlined to a few dispensing steps. Volume miniaturization to 10 mL and HTRFâ correction for media and compound interference make these kits particularly economical and well adapted to HTS and downstream screening steps.