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Member since: 2017
Effective product and good value
Application Area: Organoids development
"Significantly cheaper than other well-known names for extracellular matrices and highly effective; there are few options available on strength. Organoids develop and proliferate well pre and post-passage. Be aware it may contain traces of androgen, not specified."
Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. Basement membranes are degraded and regenerated during development and wound healing. They not only support cells and cell layers, but they also play an essential role in tissue organization that affects cell adhesion, migration, proliferation, and differentiation. Basement membranes provide major barriers to invasion by metastatic tumor cells. Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. The extract gels at 37°C to form a reconstituted basement membrane. The major components of BME include laminin, collagen IV, entactin and heparin sulfate proteoglycan. BME can be used in a multiple applications, under a variety of cell culture conditions, for maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle and stem cells. BME can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion and in vivo tumorigenicity assays. Recently we have developed an additional formulation of Cultrex® BME known as Cultrex® BME Type R1. Cultrex® BME Type R1 provides a proprietary formulation that has higher tensile strength when compared to our other products Cultrex® BME, Cultrex® BME Type 2 and Cultrex® BME Type 3. Our new Cultrex® BME Type R1 has a higher concentration of entactin, one of the BME components that connects laminins and collagens reinforcing the hydrogel structure. Cultrex® BME Type R1 has been specifically designed to culture tissue organoids