Corning® BioCoat™ Poly-D-Lysine Small-Volume 384-well Black/Clear Flat Bottom Microplate, 5/Pack, 50/Case by Corning Life Sciences

Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of many central nervous system (CNS) primary cells in culture. As PDL and PLL are synthetic molecules, they do not stimulate biological activity in the cells cultured on them, and they do not introduce impurities carried by natural polymers.Applications include:Attachment and spreading of a variety of cell typesCell differentiation and neurite outgrowthAttachment of fastidious transfected cell linesSupport survival of primary neurons in cultureSerum-free or reduced-serum cultureSourcePDL, synthetic (MW 75-150 kD)PLL, synthetic (MW 30-70 kD)Quality ControlTested for ability to promote firm attachment of RCG cellsTested and found negative for bacteria and fungiStorage4°C to 30°C under dry conditions.