CFX Connect™ Real-Time PCR Detection System with Starter Package by Bio-Rad

Manufacturer Bio-Rad  |   Model: 1855200
4.4
/
5.0
  |  9 reviews
Two-color real-time PCR detection system, includes CFX Connect Thermal Cycler chassis, CFX Connect Optical Reaction Module, CFX Manager™ Software, qbase+ Software license, cables, reagents, consumables


CFX Connect™ Real-Time PCR Detection System with Starter Package by Bio-Rad product image
CFX Connect™ Real-Time PCR Detection System with Starter Package
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Average Rating: 4.4
9 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2018

  • Organization: Nelson Mandela University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Will not buy another qPCR machine other than BioRad.
Rating: 4.7

  • Application Area: Gene quantification

"Easy to use, high quality technical assistance, and reliable. Our machine came is upgraded on a regular basis (therefore supports analyses of results). Biorad also gave a high quality workshop (with principles for publications)."

Review date: 17 Jan 2019 | CFX Connect™ Real-Time PCR Detection System with Starter Package
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Case Western Reserve University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great machine. Great service by Bio-Rad staff!
Rating: 5.0

  • Application Area:Gene expression analysis

"Bought the machine to replace old cycler. Sales staff were awesome, cooperative and on top of their things. Service was fast and efficient. They scheduled a software training for the whole lab and went over the process. The machine arrived on time and works awesome! Very happy with the cycler and the staff we worked with."

Review date: 25 Oct 2018 | CFX Connect™ Real-Time PCR Detection System with Starter Package
  • Status:

    Expert Reviewer

  • Member since: 2016

  • Organization: Charles University in Prague



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Simple to use and great results!
Rating: 4.7

  • Application Area:Gene expression analysis

"We bought this instrument to substitute an old Bio-Rad fluorescent system. The instrument and especially the software are very simple to use, very customizable. The course with the Bio-Rad technician was very useful to understand all the features of the CFX system. We use it to analyze gene expression using SYBR Green technology. The instrument is equipped with a LED lamp that doesn't need the same maintenance as the old fluorescent lamp."

Review date: 27 Mar 2017 | CFX Connect™ Real-Time PCR Detection System with Starter Package
  • Status:

    Advanced Reviewer

  • Member since: 2015

  • Organization: University College of Medical Sciences (University of Delhi)



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Without this instrument we are not published any paper from our lab after its purchase.
Rating: 4.7

  • Application Area:Gene expression study in various disease to establish disease etiology

"Bio-Rad real-time PCR machine is awesome in performance and handling. Very user friendly software. I have been using it for 2 years and to date, I face neither any problem or any complaint by my colleagues. In our lab this is one of the most important instrument which on six days a week. "

Review date: 03 Dec 2015 | CFX Connect™ Real-Time PCR Detection System with Starter Package
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: BYU



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:determine RNA expression in tissues and cells

"very useful, easy to use and easy to manage for different preparations and projects."

Review date: 10 Nov 2015 | CFX Connect™ Real-Time PCR Detection System with Starter Package
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: Reliance Lifesciences Pvt Ltd



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7

  • Application Area:Quantification of host cell DNA & Genotyping

"Very Robust and user-friendly system with options for multi tasking. After sales support is very good. The results are reproducible and consistent with very easy to interpret interface. It is a total value for money instrument."

Review date: 31 Mar 2015 | CFX Connect™ Real-Time PCR Detection System with Starter Package
  • Status:

    Advanced Reviewer

  • Member since: 2012

  • Organization: Fidia Pharmaceuticals



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:Molecular Biology

"This is an easy-to-use, straightforward PCR thermal cycler widely used among molecular biology laboratories throughout the world. Whether you are performing gene expression analyses or copy number variation assays, with the CFX96 you can produce results in less than 30 minutes thanks to the fast thermal cycling; minimize sample and reagent usage, obtaining optimal results with sample volumes as low as 10 microliters, and analyse your results when and where you want with the software which can send an email notification with attached results when a run is finished. I want to highly recommend this product as a powerful tool for your genomic research, providing the reliability you need to boost your discovery! "

Review date: 22 Apr 2014 | CFX Connect™ Real-Time PCR Detection System with Starter Package
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Korea University



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0

  • Application Area:Quantification of mRNA

"This machine is good enough for me to use it, and so strongly recommended to other groups."

Review date: 18 Mar 2014 | CFX Connect™ Real-Time PCR Detection System with Starter Package
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: University Georgia



  • Ease of use
    3 out of 5
    After sales service
    2 out of 5
    Value for money
    3 out of 5
Rating: 2.7

  • Application Area:Real-time PCR, qPCR

"Overall the instrument is handy but it does not allow you to run all samples amplified with primers tagged with all types of fluorophores. For example I cannot detect signal from PCR with Cy5 tagged primers. I would recommend making sure it filters for your choice of fluorophores."

Review date: 31 Jan 2013 | CFX Connect™ Real-Time PCR Detection System with Starter Package

Manufacturer's Response

"The three channel CFX Connect optical hardware is specifically designed to detect up to two fluorophores per well within an excitation/emission spectral range between 450-580 nm. This spectral range was selected to offer an affordable instrument that supports the most commonly selected dyes for duplex experiments and single color experiments using intercalating dyes. The spectral range for Cy5 is located in the far red spectrum. For greater flexibility in fluorophore selection and the ability to accommodate Cy dyes, the CFX96 Touch system offers the ability to detect the full spectrum of fluorophores from 450-730 nm.
Warmest regards, Rachel Scott"

Use the CFX Connect Real-Time PCR Detection System for two-target analysis. This package includes the CFX Connect Thermal Cycler Chassis, CFX Connect 96-Well Optical Reaction Module, SYBR® Green Supermix, 96-well PCR plates, and plate seals. The 96-well reaction module offers excellent thermal performance and uniformity across the entire block. Multiple data acquisition modes, including a fast-scan option, provide the flexibility to meet all your real-time PCR needs.

Features and Benefits

  • Designed for control by remote personal computer (up to 4 CFX Systems)
  • Detection of up to 2 targets per well, plus a channel dedicated to singleplex FRET
  • Integrated solution for your SYBR® Green or EvaGreen and duplex experiments
  • Thermal gradient feature that identifies optimal annealing temperature in a single run
  • CFX Maestro Software and qbase+ Software for reliable validation, data analysis, and export of RDML files for conformance with MIQE guidelines

The CFX Connect Real-Time PCR Detection System features six independently controlled thermal electric units, providing even, precisely controlled temperatures at all times during the run, including ramping. The reduced mass of the honeycomb block provides fast ramping and reduced settling time (time to achieve thermal uniformity). The programmable thermal gradient feature allows a temperature gradient of up to 24°C across the reaction block at any step in a protocol.

The optical system, with three filtered LEDs and three filtered–photodiodes, collects data from all wells, detecting up to two targets per well. For single-color FAM, EvaGreen, and SYBR Green I assays, the fast-scan option reads single-channel fluorescence in all 96 wells in just 3 seconds. A separate channel with an LED filter–photodiode combination is dedicated to FRET singleplex experiments.

Product Components

  • CFX Connect Thermal Cycler Chassis
  • CFX Connect 96-Well Optical Reaction Module
  • USB cable
  • CFX Maestro Software
  • Instruction manual
  • qbase+ Software license
  • SsoAdvanced™ Universal SYBR® Green Supermix ( 1725270 )
  • Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates ( HSP9601 )
  • Microseal® 'B' Adhesive Seals ( MSB1001 )

Configuration Options

The CFX Connect Real-Time PCR Detection System can be configured with other PCR instruments so that it can be software controlled. Instrument permutations allow up to four different CFX Connect, CFX96™, or CFX384™ Systems to be controlled by one PC. You can start the same experiment on all four instruments at once, or start a run on one instrument while another is running a different program.

Combine any two CFX Real-Time PCR Detection Systems with the CFX Automation System II , our robotic plate handler, to create a high-throughput, automated real-time PCR system with a compact footprint.

Service Plans and Validation Tools for CFX Systems

  • Ensure that your real-time PCR systems are functioning optimally with Bio-Rad Expert Care Service ; choose from several service plans to keep your CFX Automation System II running its best
  • Certify system installation and validate system performance with CFX System IQ/OQ Services and CFX Automation System II IQ/OQ Services
  • Verify the performance of your CFX Systems with Thermal Validation Services and the CFX Qualification Plates

Related Products

  • PX1™ PCR Plate Sealer
  • PCR plastics optimized for CFX Real-Time PCR Detection Systems:
    • Hard-Shell 96-Well Low Profile Skirted PCR Plates including barcoded plates ( HSP9955 )
    • Hard-Shell 384-Well Skirted PCR Plates including barcoded plates ( HSP3905 )
    • Microseal 'B' Adhesive Seals ( MSB1001 )
    • Optically Clear Heat Seals ( 1814030 )
  • CFX Maestro Software, Security Edition , for compliance with U.S. FDA 21 CFR Part 11 regulations
  • Security Edition, for compliance with U.S. FDA 21 CFR Part 11 regulations
  • CFX Qualification Plate for performance verification, 96-well ( 1845098 )
  • CFX Qualification Plate for performance verification, 384-well ( 1845099 )

Related Categories

  • Real-Time PCR Detection Systems
  • PCR Reagents and qPCR Reagents
  • PCR Plastic Consumables
  • PCR Seals
  • PCR Analysis Software

Online Resources

  • Real-Time PCR Detection Systems Selection Chart
  • qPCR/Real-Time PCR
  • qPCR Instrumentation