Member since: 2017
Organization: Newborn Screening Ontario- Children's Hospital of Eastern Ontario
Easy to design and use and the best value out there!
Application Area: diagnostic testing
"The probes are easy to use and the best value on the market. You can scale up the production size by contacting LGC and they are more than happy to synthesize any size you need and have it delivered in a timely fashion. I would like to see the BHQplus PCR probes come is a larger selection of fluorophores similar to their dual-labeled probes. In multiplexes we so see some spectral overlap between the FAM and Cal Fluor orange even with spectral calibration of the instrument which when you are looking for an absence of signal is very difficult to interpret."
The BHQplus® probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye. What differentiates the BHQplus probe from other traditional probes is that it permits the design of shorter oligonucleotides, typically 15 - 25 bases in length, for detecting more difficult target sequences.
The enhanced specificity of BHQplus probes make them perfect for genotyping single nucleotide polymorphisms.