Affinity plus qPCR probes by Integrated DNA Technologies Inc.

Use Affinity Plus qPCR Probes for SNP genotyping, transcript variant identification, and more sensitive target detection in challenging samples (FFPE tissue, biofluids). The Affinity Plus bases used in these qPCR probes are locked nucleic acid monomers. When incorporated into a probe, they impart heightened structural stability, leading to increased hybridization melt temperature (Tm).


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Affinity plus qPCR probes
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Locked nucleic acid bases, such as Affinity Plus monomers, can be incorporated into effective qPCR probes. Because locked nucleic acid bases significantly increase Tm, Affinity Plus qPCR Probes can be designed with shorter lengths than standard qPCR probes. Shorter probes have better quenching and a higher signal-to-noise ratio and are, therefore, more sensitive.