Choose this versatile midi Criterion XT Tris-Acetate Gel for denaturing or nondenaturing PAGE. The gels are made without SDS, which allow the sample buffer and running buffer to determine the separation mechanism.
Under denaturing conditions, with the SDS-containing Tricine running buffer, the discontinuous acetate and Tricine ion fronts form moving boundaries to stack and then separate proteins by molecular weight. During native electrophoresis, where proteins are separated by mass-to-charge ratio, discontinuous acetate and glycine ion fronts form moving boundaries to stack and separate proteins by both size and charge.
Criterion XT Tris-Acetate Midi Protein Gels are available as 3–8% (separation range ~40–400 kD) or 7% (separation range ~35–250 kD) gels, with a selection of different well configurations.