The AptaFluor™ SAH Methyltransferase Assay is an enabling new high throughput screening (HTS) assay to aid researchers in their search for breakthrough drugs targeting epigenetic pathways.
Methyltransferases have become a major focus of drug discovery for cancer and other diseases driven by epigenetic factors. BellBrook Labs’ new, ultra-sensitive AptaFluor™ SAH Methyltransferase Assay eliminates cost and sensitivity barriers that are slowing the discovery of methyltransferase inhibitors.
Methyltransferases produce a variety of methylated products, but most use S-adenosylmethionine (SAM) as the methyl donor, leaving S-adenosylhomocysteine (SAH) as a common reaction product. BellBrook scientists engineered a naturally occurring SAH aptamer to produce a fluorescent signal on binding to SAH, resulting in a non-radioactive assay with unparalleled sensitivity.
By directly detecting SAH, the new AptaFluor assay is universal and can be used with virtually any methyltransferase enzyme. The assay utilizes a simple mix-and-read format with a far-red positive TR-FRET readout, ensuring its compatibility with automated dispensing equipment and plate readers commonly used for HTS.