CRISPR/Cas9 is a genetic manipulation method, and the development of this revolutionary genome editing tool has enabled scientists around the world to make more accurate edits quickly and more cheaply than previously thought possible.
Like many of the molecular biology techniques, CRISPR/Cas9 is reliant on tedious 96 and 384 plate preparation, requiring consistent pipetting across microplates which can be prone to human error. To carry out effective gene editing research, therefore, it is essential that you have the right tools to hand, and Gilson has prepared a guide to help scientists maximize the levels of reproducibility in their workflow.
Cell culture is an important step in the CRISPR/Cas9 workflow and fast culture is required to maximize the probability of detecting the modification of interest. Whilst manual multichannel pipettes can be used to do this, with such large-scale workflows, manual sample preparation can often prove inefficient, time-consuming and laborious. By choosing to utilize an automated pipette such as the Gilson PLATEMASTER®, researchers have the ability to streamline their CRISPR/Cas9 studies at scale.
The PLATEMASTER boasts both high levels of accuracy and an intuitive operation system. The system works by reducing the number of pipetting steps necessary to fill a microplate (when compared with other regular multichannel pipettes), and it is possible to fill an entire 96-well plate in less than 10 seconds.
The PLATEMASTER is considered an ideal solution for performing both 96-well and 384-well plate-to-plate transfers or for transferring buffers or media from a reservoir. It also reduces the risk of human error, by eliminating any chance of skipping or repeating wells by accident.
The system requires zero programming and little set-up is required. This, along with its small footprint, makes it ideal to use in a laminar flow hood or cabinet and for moving around the lab as needed.
The PLATEMASTER has received positive feedback from scientists leaving reviews on the SelectScience® website. Ana Kitanovic, of IFM Therapeutics, uses the instrument for compound screening, supernatant collection and HTRF assays. She describes it as an easy-to-use instrument that can save a lot of time and says it has significantly increased screening throughput. The reviewer also highlights the "very good price," concluding:
A must have instrument for all screening labs!
Gilson provides liquid handling solutions for each stage of the CRISPR/Cas9 workflow, download this guide to find out more.