Application Note: Quantitative Measurements of Apoptosis using the NovoCyte Flow Cytometer
12 October 2015
Apoptosis, or programmed cell death, is an active process by which cells regulate how they die by triggering specific pathways that cause the cell to shrink, condense, and eventually be cleared by phagocytosis. This is in contrast to necrotic cell death where cells die uncontrollably due to external factors and fall apart, leading to detrimental effects such as the activation of an immune response. Therefore, apoptotic cells that die in a very orderly fashion have no or limited disruption of nearby cells and tissue. This application note demonstrates three different assays that can measure apoptosis in response to staurosporine, a broad kinase inhibitor known to induce apoptosis in many cell types.