Application Note: Protein Separation with a new High Resolution Glass Column
3 May 2011
In this application note by KNAUER albumin is purified from myoglobin using a size-exclusion chromatography method. A new glass column allows optimum pressure filling up to 15 bar (1.5 MPa) and safe operation up to 100 bar (10 MPa) in the glass tube, thus the operating range of the biochromatography medium can be extended. The pressure resistant filling funnel of the column allows the gel to be filled under pressure from the outset while the column bed can be further compressed after the upper adjustable adapter is placed into the glass tube.