Application Note: Peptide Microarrays for Epitope Mapping of Antibodies Against hTSHR
24 August 2017

In order to use antibodies as diagnostic markers for the prediction of both prognosis and therapeutic response, accurate characterization of their antigen binding region is indispensable. This application note presents the use of a peptide microarray for the diagnosis and epitope mapping of human anti-thyrotropin receptor (hTSHR) antibodies. In the study, the peptide microarray exhibited excellent performance in single and multiplex antibody analysis as well as high specificity, giving the technology potential as a multi-determinate in vitro diagnostic assay for autoimmune diseases.