Application Note: Novel Methods to Analyze Platelet Activation with NovoCyte Flow Cytometer
9 May 2018
Due to the cumbersome and error-prone nature of light transmission aggregometry (LTA), improved methods to measure platelet function are needed. Measuring platelet activity by flow cytometry offers several advantages over LTA in that only small amounts of blood volumes are needed, whole blood can be used, and results are independent of platelet count. Furthermore, the possibility of ready-made tubes with fixative would allow more time between blood collection and analysis, which is crucial for field testing. In this application note, researchers use the NovoCyte® flow cytometer to measure platelet function in two ways: expression of CD62P and frequency of platelet-leukocyte aggregates.