Application Note: Measuring Mouse GFR by FITC-Inulin Using the Thermo Scientific NanoDrop 3300 Fluorospectrometer
14 January 2015

The measurement of glomerular filtration rate (GFR) is the gold standard in kidney function assessment, and as such is used as an indicator of kidney disease. The method described in this application note demonstrates the feasibility of non-radioactive and repeated determination of GFR in conscious mice without surgical procedures or metabolic cage experiments; it uses a microvolume FITC-inulin procedure for use on the NanoDrop 3300 Fluorospectrometer.