Application Note: MACSQuant Flow Cytometers - FRET Express Mode
7 February 2019
Fluorescence resonance energy transfer (FRET) is a mechanism describing an energy transfer between two fluorescent molecules. The efficiency of this energy transfer is inversely proportional to the distance between donor and acceptor, making FRET extremely sensitive to small changes in distance. Measurements of FRET efficiency can be used to determine if two fluorochromes are within a certain distance of each other. Therefore FRET can be used to detect molecular interactions in a number of systems and has hence often been applied in various assays in biology and chemistry.