Application Note: Label Free Mitotic Index
24 April 2017

Current methods that exist to measure mitotic index usually rely on the use of fluorescence markers and relatively high light levels, which can ultimately perturb the natural function of cells.  This application note demonstrates the capability of the Phasefocus system to image non-invasively and identify label free unique phase signatures that indicate when a single cell is undergoing mitosis. The nature of the continuous measurement allows the mitotic index to be extracted at every time point, and subsequently a more robust and true representation of the behaviour of the cells to be categorised.