Application Note: Inhibition of T Cell Receptor Signaling Mediated Calcium Flux
3 May 2018

Flow cytometry has the unique capability to determine Ca2+ levels in several millions of individual cells over time as well as in an entire population. Intracellular Ca2+ measurements utilize fluorescent Ca2+ indicators which exhibit an increase in fluorescence upon binding to Ca2+. Ca2+ indicators such as Quin-2, Fura-2, Indo-1, Fluo-3, Fluo-4/AM can be used to quantify Ca2+ concentrations via flow cytometry. This application note describes a technique that utilizes the NovoCyte® flow cytometer to measure calcium flux in a cultured T cell line and assess the inhibition of T cell receptor (TCR) -mediated calcium flux by ibrutinib.