Application Note: Human Blood Lymphocyte Subset Analysis with Lyse/no-wash Method
8 September 2015

Blood is comprised of complex populations of lymphocyte, monocyte, granulocyte, platelet and red blood cells. Flow cytometry represents the best method for studying functional and phenotypic properties of these sub-populations. Based on biological function and cell-surface antigen expression, lymphocytes can be divided into three major populations, ie, T lymphocytes (CD3+), B lymphocytes (CD19+), and NK lymphocytes (CD16+CD56+). CD3+ T lymphocytes can be further divided into CD4+ T helper/inducer and CD8+ suppressor/cytotoxic subpopulations. This application note demonstrates the use of the NovoCyte flow cytometer for the analysis of human blood  lymphocyte subset.