Application Note: HPV E6/E7 mRNA Expression Analysis Using the NovoCyte Flow Cytometer
27 November 2018

Widespread screening has been extremely effective for the treatment and prevention of cervical cancer, however, cytology methods of cervical screening require highly trained cytopathologists and do not diagnose HPV infection. Since the discovery of HPV as the underlying cause of cervical cancer, morphologically abnormal cervical cells have been routinely screened for the presence of HPV DNA by PCR analysis. HPV DNA analysis in cervical cancer screening has increased the detection of high-risk lesions with normal cytology results and decreased unnecessary biopsies in women with atypical squamous cells of undetermined significance (ASC-US).