Application Note: Direct PCR from Mouse Tissue using Phire™ Hot Start DNA Polymerase and Piko™ Thermal Cycler
6 June 2008

This application note demonstrates how Finnzymes’ Phire™ Hot Start DNA Polymerase enables robust DNA amplification directly from a variety of mouse tissues without prior DNA extraction or purification. When combined with a fast PCR instrument such as the Piko™ Thermal Cycler, Piko™ PCR Plates (Finnzymes Oy), and the FlashGel® system (Lonza), protocols can be performed in 30-40 minutes (from sample to gel image) and with reaction volumes as low as 5 μl.