Application Note: Direct Enumeration of CD34+ Cells in Blood Using the NovoCyte Flow Cytometer
30 November 2018
Hematopoietic stem cell transplantation (HSCT) is an important clinical procedure used for treatment of blood malignancies and disorders. The majority of HSCTs performed use peripheral blood as the source of hematopoietic stem and progenitor cells (HSPC) and success of hematopoietic stem cell transplantation is associated with the quality of progenitor cells infused. Successful long-term hematopoietic transplantation highly correlates with the number of CD34+ HSPCs transferred. Therefore, CD34+ cell counts are used as a quality control for transplantation and CD34+ enumeration is essential for HSCT.