Application Note: Differential recovery of gabapentin and pregabalin
7 August 2019

As demand for therapeutic drug monitoring rises, methods that can be used to quantify a wide variety of drug chemistries from a single analysis are increasingly being implemented, as they reduce costs and streamline workflows. In this application note, Tecan outlines how solid-phase extraction (SPE) can be utilized in a 43-analyte urine confirmatory LC-MS/MS panel. Here, Tecan demonstrates how the concentration and types of organic solvents used in the elution step can be modified to predictably and reproducibly reduce the recovery of gabapentin and pregabalin without affecting the complete recovery of other analytes. This differential recovery can reduce the complications associated with samples with high concentration gabapentin/pregablin such as offscale chromatography, carry over, and ion suppression.