Application Note: Detection and Quantitation of Eosinophils with Other Leukocyte Subsets Using the NovoCyte Flow Cytometer
29 March 2016

Eosinophils play an important role in the immune system, playing an essential role in inflammatory responses and allergic reactions as well as combating parasitic infections. Traditionally eosinophils are quantified by manual counting though this method involves long sample preparation and can be influenced by human error. This application note describes a flow cytometry-based assay used at the National University of Singapore using the NovoCyte Flow Cytometer from Acea Bioscience, in order to accurately identify and quantify eosinophils in human peripheral blood.