Application Note: Cell-Based Screening of Drug-Induced Metabolic Dysfunction
28 January 2019

This poster highlights the compatibility of such advanced cells models with the MitoXpress Xtra Oxygen Consumption Assay which facilitates direct microplate-based analysis of mitochondrial function measured on a time-resolved fluorescence enabled plate reader. The assay is successfully applied to both HepaRG cells and to Primary Hepatocytes, while, using the RAFT™ system, mitochondrial function of cells within 3D collagen constructs can be assessed. To better understand the metabolic consequences of drug treatment, measurements are made under both basal and uncoupled conditions such that any diminution of aerobic metabolic capacity can be better detected.