Application Note: Assessing mitochondrial dysfunction in primary cardiomyocytes
20 May 2014

This Application Note describes a method for proling mitochondrial function in cells responding to
stress. The mitochondrial prole generated in this way provides four parameters of mitochondrial function
that can be measured in one experiment: basal respiration rate, ATP-linked respiration, proton leak,
and reserve capacity. The results described here suggest that the development of cardiomyocyte injury,
in this case caused by an oxidized lipid, increases ATP-linked oxygen consumption, diminishes respiratory
efciency, and depletes the bioenergetic reserve capacity.