Application Note: A Systematic Approach Towards UPLC Methods Development
11 June 2008

By utilizing UltraPerformance LC (UPLC) Technology for methods development, a 6-fold improvement in throughput can be realized. This, in turn, reduces cost per sample and time of analysis considerably while maintaining or improving separation integrity. By developing rapid, high resolution analytical methods, products can be brought to market faster, therefore, improving the overall profitability of the assay.

In this application note, combinations of selectivity factors (pH, column chemistry and organic modifier) in UPLC separations were examined to develop high resolution chromatographic methods for separating paroxetine hydrochloride and its related compounds.