Application Note: Screening for drug-induced mitochondrial dysfunction using MitoXpress Xtra on isolated mitochondria
20 January 2020
Oxygen consumption measurements have been the preferred method of assessing mitochondrial function. However, the measurement complexity and low-throughput nature of traditional polarographic Clark-type electrode approaches have limited the applicability of these informative measurements, particularly in the context of compound screening. In this application note, Agilent Cell Analysis describes how the MitoXpress Xtra Oxygen Consumption assay is designed to address this deficit, facilitating a fluorescence-based high-throughput interrogation of mitochondrial oxygen consumption in 96- or 384-well plate formats.