Application Note: Quantify meaningful changes in the metabolic status of single cells
18 October 2021
Metabolic imaging based on fluorescence lifetime provides insights into the metabolic dynamics of cells, but its use has been limited as expertise in advanced microscopy techniques was needed. Now, STELLARIS 8 DIVE FALCON makes metabolic imaging accessible to every scientist, thanks to the integration of phasor analysis to easily use fluorescence lifetime information.
FLIM phasor analysis is a robust analytical method to generate a visual fingerprint of the lifetime of a fluorophore that can be mapped back onto an image to highlight function in cells. Download this application note for a summary of studies that use FLIM phasor analysis to reveal connections between cellular metabolism and pivotal cellular functions in health and disease.