Application Note: Quantification of Purified PCR Samples
16 October 2017
This note describes how to use the purified PCR application on the Lunatic systems. This Unmix application is used to analyze the UV/Vis spectral shape of the sample to isolate the fraction of the molecule of interest from co-absorbing entities contributing to the total UV/Vis absorption spectrum. Accurate quantification of the molecule of interest is established using its isolated spectrum fraction.