Application Note: Oligo Pools: Design. Build. Test.
26 June 2019

Oligo pools, high diversity collections of oligonucleotides, are utilized in many applications, including generation of CRISPR single guide RNA (sgRNA) libraries and high-throughput reporter assays. Accuracy and uniformity of oligo synthesis are critical to ensure the oligo pools that are generated precisely match the design. Oligo pools for CRISPR screens cloned into expression vectors generate sgRNA libraries targeting loci of interest. Twist Bioscience’s industry-leading low synthesis error rate and uniform sequence representation result in high quality libraries that enable the most efficient CRISPR screens.