Application Note: Off to the Races: Rapid Buffer Exchange, Protein Quantification and Stability Screening with Junior, Lunatic and Uncle
19 September 2018

The conformational, chemical and colloidal stability of a protein are strongly influenced by the buffer solution. Altering buffer salts, pH, ionic strength, excipients and surfactants may increase or decrease the stability of a molecule. The process of screening formulation buffers to optimize stability is a time consuming process. The time required to perform buffer exchange is often a limiting factor in biologics development. Junior with buffer exchange removes the buffer exchange bottleneck by automating buffer exchange and sample concentration, which reduces required hands-on time and increases the number of samples that can be prepared in a day.