Application Note: Highest Precision with 1.5-Fold Discrimination for qPCR Quantification Applications
11 October 2018

A valid real-time PCR assay is characterized by four major properties: PCR efficiency, reproducibility, specificity and sensitivity. Several factors have an influence on these critical points; beside primer design, plastic ware and master mix, of course the performance of the real-time PCR cycler itself affects the results. For this reason, it is mandatory to guarantee best properties of the real-time PCR cycler to rely on the outcome of the applied real-time PCR.