Application Note: DRAQfx™ in Immunofluorescence
19 January 2018
Immunofluorescence (IF) microscopy often involves the analysis of adherent cells or tissue sections where samples have been preserved with formaldehyde-/formalin-fixation (FF). Tissue samples can be paraffin– embedded (FFPE) or snap-frozen. After sectioning they are processed to enable analysis, which might include dewaxing, rehydration, antigen retrieval and permeabilized with weak surfactant to make cells permeable to antibodies. Then, fluorescently-tagged antibodies can be used to label internal structures in adhered cells or thin tissue sections. It is important to view any antibody staining of cells in the context of an individual cell and its internal structure as well as any adjacent tissue morphology.