Application Note: Channel- & Compensation-free Dead Cell Exclusion: N+1 Panel Design
19 January 2018
Dead cell exclusion is a common requirement for flow cytometry using viability dyes like DAPI, PI and 7-AAD. Anew far-red fluorescing alternative DRAQ7™ has been robustly tested showing improved separation between negative and positive events. Conventional flow cytometry is limited by spectral "space" and the preference for bright chromophores to label antibodies to aid sensitivity that increases channel spillover. At its simplest, far-red DRAQ7 can benefit panel design where, for example, the complete absence of spillover into the FITC and R-PE detectors makes it an obvious replacement for 7-AAD in CD34+ cell enumeration.