Application Note: A Fast Dilute-And-Shoot Method for Simultaneous 5-Hydroxyindoleacetic Acid (5-HIAA), Vanillylmandelic Acid (VMA), and Homovanillic Acid (HVA) LC-MS/MS Analysis in Human Urine
7 June 2017

In this partial validation study, the performance of a fast dilute-and-shoot LC-MS/MS method is demonstrated using a Raptor™ Biphenyl column for the simultaneous analysis of 5-hydroxyindoleacetic acid (5-HIAA), vanillylmandelic acid (VMA), and homovanillic acid (HVA) in human urine. Excellent results were obtained for linearity, accuracy, and precision. With this method, these serotonin and catecholamine metabolites can be measured in human urine with a 5-minute total analysis time. The analytical method is applicable to clinical labs that require a combination of fast analysis times and low-cost sample preparation procedures for 5-HIAA, VMA, and HVA using LC-MS/MS analysis.