Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.Based on immunoelectrophoresis, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human immunoglobulins. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
|Format||Antisera to Immunoglobulins|
|Storage||Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.|
|Buffer||0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6|
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JIR anti-Fc robust and stable capture!
Application Area: Capture reagent
"The anti-human polyclonal capture reagents are some of the most robust reagents I have in my biophysical and biochemical studies so far. The capture is very stable for a long time (hours) in an experiment, the anti-human coated chips/plates can be re-used multiple times (sometimes for weeks to a month) without losing antibody capturing capacity and chip surface integrity. Overall, the anti-Fc reagents regardless of the host or capturing species provides a robust capturing ability with minimum cross-reactivity to other species."