Direct-Blot™ HRP anti-β-actin; Clone: 2F1-1; Reactivity: Human, Mouse; Apps: WB; Size: 100 μl (20 Western blots)
Actin, cytoplasmic 1
|Isotype||Mouse IgG2b, κ|
|Quantity||100 microl (20 Western blots)|
|Western Blotting (WB)|
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Member since: 2018
Organization: Baylor College of Medicine
Performs well with clear results
Application Area: Western Blot
"We used this antibody as a faster method to see our protein loading concentrations. Instead of incubating overnight, we incubate for 1 hr at room temp. The sensitivity and specificity were comparable to our other actin controls (overnight incubation with secondary HRP antibody). We used a 1:80000 dilution with about 20 micrograms of protein loaded."
Member since: 2017
Organization: UC Sandiego
Performs well with no any viriability
Application Area:Western blot
"This antibody is easy to handle, it has decent sensitivity, we use them as a control to test in vivo protein degradation, the result is clean and clear."