EGR family members are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression. The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF. Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation.
|Reactivity||Human, Mouse, Rat|
|Storage||Store at –20°C. Do not aliquot the antibody.|
|Buffer||Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide|
|Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P)), Immunoprecipitation (IP), Western Blotting (WB)|
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Member since: 2015
Organization: State University of New York At Buffalo
Application Area: Western Blot
"The antibody worked great at a concentration of 1:500 on 20ug of brain tissue. It does leave some unspecific bands but there is enough separation to distinguish between EGR isotopes."