All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Acetyl-Histone H3 (Lys27) set includes the Anti-acetyl-Histone H3 (Lys27) antibody, a negative control antibody (purified Mouse IgG), and qPCR primers which amplify a 178 bp region within the promoter of the human RPL10 gene. The acetyl-histone H3 (Lys27) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of acetyl-histone H3 (Lys27)-associated chromatin.
H3K27Ac; Histone H3 (acetyl K27); Histone H3K27Ac
|Storage||Stable for 1 year at -20°C from date of receipt. Aliquot upon thawing, avoid freeze thaw cycles.|
|Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)|
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Member since: 2018
Organization: Brigham and women's hospital
Antibody with high quality.
Application Area: ChIP and ChIP-seq
"I always achieve reproducible results for ChIP experiments with this antibody. It is reliable, and easy to use. It contains primer set as negative control and positive control."