Comparative Analysis of FFPE-Derived RNA: A Novel Relationship Between RNA Quality and Sequencing Results
18 Dec 2017

RNA derived from formalin fixed, paraffin embedded (FFPE) tissues are invaluable for gene expression analysis of a myriad of disease states. However, the process of fixing the tissue and embedding it in paraffin severely affects the functionality of the RNA in downstream assays. These challenges are compounded by the labile nature of RNA and the ubiquitous presence of RNase that makes RNA difficult to work with throughout the sample preparation process.

The advent of robust next-generation sequencing (NGS) technologies has enabled low quality and low input RNA for RNAseq workflows. Nevertheless, the reliability and accuracy of RNAseq data is still dependent on the extent of template RNA quality and amount.

This educational webinar will detail the significant impact of RNA quality on RNAseq fusion call sensitivity as a function of RNA extraction. Interrogating the FFPE derived RNA quality is a critical and yet overlooked step towards achieving optimal NGS results. It will also discuss proper conditions and parameters that should be taken to overcome some of the challenges in FFPE extractions.

Webinar attendees will learn about:

  • The differences between high, low and medium quality RNA
  • The types of RNA quality metrics and methods of analysis used today
  • Why it is difficult to isolate good quality RNA from FFPE tissues
  • The importance and effects of RNA quality and extraction methods in RNAseq workflows and outcomes
  • Countermeasures to optimize FFPE sample preparations to enable successful downstream assays

Beckman Coulter

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