Solving the Aggregation Problem of Cells in Suspension Culture
01 Sep 2015

Mammalian cells have become the dominant system used to develop biopharmaceuticals, due to their ability to properly fold and assemble proteins and add post-translational modifications such as glycosylation. Growing them in suspension culture or adapting them to suspension allows for higher yields required for downstream processing. However, some cells, when grown in suspension, start to clump together and there are many solutions available commercially to combat this problem.

In this webinar, an efficient new method that was used to develop aggregation-free HEK-293 cells in suspension from attachment culture is discussed by Stacey Willard, a Senior Scientist at Eppendorf.

By watching this webinar, you will learn:

  • A basic understanding of culturing cells in suspension, including the advantages and disadvantages.
  • A novel approach to solving cellular aggregation that occurs when culturing certain cells in suspension.
  • Tips on how to adapt this method to increase the overall efficiency of your cell line development process.

Eppendorf