Stewart Rudnicki

Job title: Laboratory Manager / Supervisor

Discipline: 
Work field: Academia / University
Organization: Harvard Medical School - ICCBL

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    CyBio® Well Vario
    "We have had our Cybi-Well Vario for more than 10 years now. It used to be our go-to instrument for library reformatting from 96-well to 384-well microplates. It takes just a couple of minutes to switch between the 96-channel and 384-channel heads which is very useful. As an accurate pipettor, it is our best in the lab. You ask for 2uL transfer, and that is what it gives you with great CVs. It is also very dependable. As one of our older instruments, we have a version that has a maximum pipetting volume of 25uL on the 384-well head. We find this to be limiting compared to some of the more recent pipetting platforms out there. It appears that they now have a 60uL head option, which would be recommended. Our other system can transfer up to 70uL with its 384-well head, and that has made it more attractive for many applications. One thing that I particularly like about the Vario is that it is user-friendly. We can have users of the facility use it, because there is little chance of damaging the unit when pressing on the tips. On our other platform, if the tips are not aligned properly, it can severely damage the head. On the Vario, that is not an issue. We used to be able to get 3rd party tips (Thermo/Matrix) for the instrument but I am not sure if these are still available. They were kind of expensive. PROS: (1) It is very accurate and precise (2) Very dependable instrument (3) Easy to use (4) Hard (but not impossible) to break. CONS: (1) 25uL pipetting volume head is quite limiting (2) It's a little slow (3) Tips are/were somewhat expensive. If you are looking for a dependable pipetting platform to run day in and day out, this is a good option."

    Application area: Small Volume Liquid Handling with Research Samples

    1 November 2017
    • Ease of use5 out of 5
    • After sales service4 out of 5
    • Value for money4 out of 5
    Average    

    4 out of 5

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    Vertical Pipetting Station
    "We bought the Velocity11 (now Agilent) VPrep (now Vertical Pipetting Station) over 10 years ago, when the Bravo was first being developed. This was a stand-in for that anticipated Bravo, but we ended up liking it so much that we bought this one as well. It is great for what it does - transfer liquid 96 or 384 channels at a time. We use ours daily and it is our in-house library reformatting instrument for small molecule libraries dissolved in DMSO. The heads are easily swapped out in 30 seconds, and the shelves move in and out underneath the head for pipetting. We are still using an old version of the software called Prepworks, but it still works great and we feel no compelling reason to upgrade. Pros: • The instrument is fast and dependable. • It has an incredibly small footprint. • The heads are easily switchable. • There are 3rd party tips for the instrument that work well and are very inexpensive. Cons: • There are only 8 positions and only 6 that can accommodate standard microplates. The bottom two are for tips or deepwell plates. • Occasionally it is unhappy pressing on 70uL 384-well tips, but a simple retry solves this. • The heads are expensive and no independent channels for serial dilutions or hit picking."

    Application area: Plate stamping, liquid handing

    26 October 2017
    • Ease of use5 out of 5
    • After sales service3 out of 5
    • Value for money4 out of 5
    Average    

    4 out of 5

    Read more


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