Raghothama Chaerkady

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    "BEH columns are useable for all pH range reversed-phase separation and helped us to replace other laborious desalting cleaning steps before LC-MS/MS experiments for peptides. We use it for iTRAQ, phosphopeptide analysis and global proteomic analysis. Peaks are evenly distributed across the gradient unlike strong cation exchange methods. Basic RPLC using BEH column is our favorite offline method. Peptide separation is very different in low and high pH condition offering a scope for better fractionation. The only one pre-step needed is 30 kDa buffer exchange of protein lysate in urea."

    Application area: Proteomics

    16 February 2012
    • Ease of use5 out of 5
    • After sales service4 out of 5
    • Value for money5 out of 5

    5 out of 5

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