Bio-Plex® MAGPIX™ Multiplex Reader (171-015001)
"We ordered the 21-plex M50-00031YV and 27-plex cytokine M50-0KCAF0Y arrays to assess levels of cytokines in human bone marrow plasma samples. We found the procedure in general easy to follow. However, during the analysis we found that despite the fact that the premixed combinations of cytokines were all pipetted at once, some of the cytokine control curves did not look well. As a result some of the cytokines could not be analysed, whereas other cytokines from the same wells and the same procedure did not encounter any problems. Since apparently all the pipetting and washing procedures went well, we firmly believe a technical error during manufacturing (or premixing of the cytokines) may have resulted in these results. From the in total 48 analysed cytokines, we encountered problems only with the following 6 cytokines: Eotaxin, IL-13, IL-15, IL-2, IL-7 and SDF-1a. All other cytokines were read and analysed appropriately."
Application area: analysis of bone marrow plasma samples
5 August 2013
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