Top List: 6 Innovations in Drug Discovery Screening

Live cell monitoring, biopharmaceutical workflows and peptide screening

Discover the latest in drug discovery screening. Watch our on-demand webinar event on the technology accelerating biopharmaceutical research and download methods for the analysis of live cells.

1. Application Note: NanoBRET™ Assay for Monitoring of Ligand Binding to GPCRs in Live Cells

G protein-coupled receptors (GPCRs) play a crucial role in mediating cellular responses to external stimuli. BRET (bioluminescence resonance energy transfer) requires only the addition of a suitable substrate to generate donor light emission, as well as energy that can excite fluorescently-labelled ligands in a non-radiative manner. This application note demonstrates the first use of BRET for monitoring ligand binding to GPCRs in live cells enabled by NanoLuc® luciferase, using the CLARIOstar from BMG Labtech. Download method.

2. Free Webinar Series: Technology Accelerating Biopharma: Complete Workflow Solutions in Biopharmaceutical Discovery and Development

Protein biopharmaceuticals are being developed at an explosive rate and have attracted great interest from both smaller biotech firms and big pharma. However, discovery and development of biopharmaceuticals is difficult. There are many challenges, and scientists must not only stay abreast of advances in knowledge and improvements in technology, but also navigate the maze of shifting government regulations. Watch the free webinar event on-demand.

3. Application Note: Multiplexed Cell-Based Assay for Antibody Screening Against Cell Surface Receptors

This application note describes the use of TTP Labtech’s mirrorball for the determination of antibody binding to human epidermal growth factor receptor (EGFR) using a “mix-and-read” assay as an example of a screen for a cell surface antigen. EGFR ligands are a family of proteins which are involved in the regulation of cell migration, adhesion growth and differentiation. Download method.

4. Top List: 6 Innovations in Drug Discovery Biomolecule Research 

Discover a smartphone app that improves column selection, plus a new method for the quantification of bradykinin in plasma. Learn more about high-tech column selection, natural product characterization and quantitation, and transferring workflows from ELISA to FLISA. Read article.

5. Application Note: Label-Free High-Throughput Peptide Screening

High-density peptide arrays have been measured with the Berthold Technologies bScreen featuring the Biametrics 1-lambda RIDe label-free technology. It is possible to screen up to 20,000 of peptide-protein interactions per cm² within a few minutes and high affinity peptide binders against the pharmaceutically interesting target growth factor receptor-bound protein 2 (Grb2) can be identified and characterized. Download method.

6. Application Note: Development of Integrated Informatics Workflows for the Automated Assessment of Comparability for Antibody Drug Conjugates (ADCs) Using LC-UV and LC-UV/MS

Antibody Drug Conjugates (ADCs) have been emerging as a new class of anti-cancer therapeutics, combining the targeted delivery capability of a monoclonal antibody (mAb) with the potency of conjugated cytotoxic agents. By releasing the cytotoxic drugs inside target tumor cells, ADCs minimize exposure of non-target cells to toxic drug payloads and thereby reduce potential side effects of the treatment. This application note demonstrates an integrated informatics workflow using UNIFI® Software, from Waters, that streamlines data acquisition, processing, and reporting for ADC analysis by LC-UV or LC-MS. Download method.

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