Zymoclean™ Gel DNA Recovery Kit by Zymo Research

"The product is highly recommended, you can separate bands in a gel when you get noise or artifacts, or only purify your PCR product for special downstream applications. The care after sale is very simple as it is stored at room temperature. The use is easy and is difficult not to obtain the expected results. I just recommend extend or repeat the last centrifugation in the wash step to eliminate any ethanol residue, and use less water or buffer TE to the end recovery of DNA in two steps of incubation and centrifugation for a higher recovery due to the fact that the columns use to retain some DNA."

"The product was easy to use and we were able to obtain high quality gDNA, we highly recommend it to researchers who are in the same line of study. There is great value for the money spent for buying the kit, though kinda pricey by our standards here, the results were satisfactory."

"Results are good. Easy to use, reproducible. Quick and fast"

"High yield, quick protocol"

"Cheap, easy to use, good results"

"A really good DNA recovery and purity in comparison with other kits in commerce. Moreover, column design permits DNA elution at high concentration into really small volumes, that is perfect for subsequent DNA ligation during cloning."

"It is a simple and high-yield process that I can save my time. However, it gives me an ethanol contaminated product by following the protocol and I added one more time of washing step and dry out by extra centrifuge after washing. Since contaminated products gave me less efficiency of further cloning steps such as ligation, I do highly recommend that you add the additional washing and extra centrifuge to remove any residue of ethanol on your protocol(a simple version of). Overall satisfaction is good and I would like to keep using this product for my experiment. Thanks!"

"I would recommend using 20ul or more at the elution step. Although, the melting step is a painful amount of waiting time."

"The product is extremely easy to use. It hasn't failed me until now. I use it for cloning and extracting PCR fragments from TAE 1x agarose gel. Yields are great and so is the purity. The kit was recommended by a friend of mine in the US as being "The best gel extraction kit.""

"The go-to gel purification kit in our lab. Simple and easy to use, we get decent yields and usually no issues with downstream applications (mostly ligation into a vector or E. Coli transformation. Can't go wrong!"

"Easy-to-use Kit. I used it a few times and have no problem following the steps."

"Reproducible results, easy and quick to use"

"Only a few steps - really easy to use. Great shelf life too,"

"We achieved high quality purification of the PCR product using this kit. It is very easy to use and reliable."

"The Zymoclean Gel DNA Recovery kit has been very reliable for cloning applications in our lab. We frequently clone with DNA and vector inserts ranging from as small as 200 bases to as large as 10 kilobases in length. This gel extraction kit enables us to reliably purify and concentrate DNA for downstream use. It is a quick and easy to use kit. I'd recommend this kit for anyone looking to isolate and concentrate DNA fragments for a variety of applications."

"It is very easy to use, yields good results."

"This kit has the amazing properties to purify the extracted gel which has the amplified product in it. Very quick to purify the gel and provides good quality and quantity. High concentration with great elution which is very helpful and required for the experiments like sequencing, and cloning. A great product to have in the scientific labs."

"Saves so much time compared to freeze-and-squeeze and provides better quantity and quality of yield compared to other methods and even other kits. For the time this saves it's really a great value. "

"The kit allowed me to finally isolate my DNA from gel. The kit is easy to use and good at what it does."

"Very easy to follow protocol, product gives high DNA yield."

"It gives very good yield, easy to follow protocol, and quick."

"Excellent gel DNA recovery kit."

"We have been using Gel DNA recovery kit for a long time. We are very happy with the yield we get using this kit. Also, its very cost effective. "

"The best to get good DNA results with minimal loss."

"Love being able to elute in a small volume - excellent for downstream applications!"

"High yields compared to other products in the market. Easy to use."

"This product is great and contains plenty of buffer."

"I have tried many gel extraction kits over the years and the Zymo DNA Gel Extraction kit is far superior. Start to finish it takes about 10 minutes from cutting the band out of the gel to having highly pure DNA in a small (6uL) volume. There isn't significant loss over the column and there are only 2 solutions to use. DNA can be eluted in water or buffer. I consistently get DNA with 260/280>1.90. I highly recommend this kit and it is the only kit we now use for this purpose in my lab. "

"We use Zymoclean Gel DNA Recovery Kit before sequencing clinical samples on an Illumina MiSeq. The instructions are clear, price is good for the amount of preps you get, and we haven't had any delivery time issues during the pandemic."

"Gel DNA Recovery Kit is our go to kit for this purpose, we have been using it for years, consistent quality and results!"

"This kit is awesome! It contains lots of buffer, so you're not limited in that like with some other companies' kits. The kit is very flexible with straightforward directions, so you can really adjust it to get the best results with your own protocols."

"Got good gel recovery and was easy to use. Minimal steps was an advantage over other brands."

"Very easy to use! The range of centrifugation instead of a set speed was new to me, but didn't seem to hinder any results."

"Of all the many gel extraction kits I have used over the years this kit is superior. Within about 10 minutes of cutting the band from the gel I have highly pure DNA in a small (6ul) volume. There are only 2 solutions and the column based approach makes everything quick and user friendly. We only use this kit in my lab and I highly recommend it."

"Very easy to use product. Columns yielded good, pure samples that were readily Sanger-sequencable."

"I use this kit for extracting DNA intended for NGS applications. It is fast, easy and I always get a good, clean recovery.`"

"After many years using Qiagen kits, I happily use the equivalent Zymo kits now, including the gel cleanup kit. Works great, lower cost."

"It is very easy to use and efficient. Good quality of extraction."

"This product is very easy to use. It takes only about 30 minutes to complete from start to finish. The instructions are easy to follow and it results in high quality DNA able to be successfully used in cloning."

"We use this for cloning PCR DNA. This is a great product and easy to use. Our favorite aspect is the small volume elution (6 ul) that allows for the concentration of dilute samples."

"Simple and easy to use."

"Its easy to use; following the steps in the protocol, It does not affect the yield of the DNA after gel recovery and clean up and I could successfully build plasmids with my PCR product recovered by this kit multiple times. Thank you so much Zymoclean for being reliable and a time saver."

"This kit is very easy to use! You can go from gel piece to purified DNA in very little time so it's great if you have a lot of samples. I use it when I'm doing restriction enzyme cloning and it works well. I would recommend this product!"

"This kit is fantastic! I had so much trouble extracting DNA in a high enough yield with competitors' kits. The Zymo Gel DNA Recovery kit is a life saver for cloning! I am continually impressed with all of the Zymo products!"

"I first tried the free sample provided by Zymo and was extremely happy with the results. Clean purification with high % yield of the fragments from the gel in order to proceed them for cloning. I purified both PCR fragment as well as restriction digested fragment. The cloning too was successful upon the use of these good quality products. I highly recommend this. We bought several boxes of it for our lab."

"It is very easy to use and I like it because you only need a small amount of water to elute the DNA. So you can get higher concentration of recovered DNA. Product quality has been very reliable that we don’t have any issue with the solution or columns."

"Overall, this is a reliable and efficient purification system from agarose gels. The small elution volume allows one to limit the material necessary for upstream processing. Yields high purity and reasonable quantity DNA that is usable for subsequent PCR, recombination cloning or traditional cloning. Highly recommend."

"Best kit in the market. Yields are much better than with Qiagen kits. The column design is what makes the difference."

"The Zymoclean Gel DNA Recovery kit was perfect for our experimental workflow. We always got very clean DNA, with maximum yield from the gel. It was extremely useful to be able to adjust the final elution volume to obtain concentrated samples. Other than making sure to add ethanol to the wash reagent, there is little that could possibly go wrong. The protocol was simple, quick, and reduced user errors effectively. We will use this product again, and I highly suggest others use it for their applications!"

"Purification of PCR and cut DNA plasmids for cloning. My favorite kit. Small volume elution of 6ul is great for low abundant PCR products and often avoids repeating the PCR to make more DNA. Very clean and great for cloning."

"In comparison with other products intended for the same use, the Zymo kit gave the best results, cleanest DNA and also highest yield. In addition to this, the supply of the sample kit was really helpful as it allowed me to compare products before purchasing the larger Zymo kit."

"This kit is quick and simple and gives you great recovery. Consistently isolates DNA from EtBr gels with no issues."

"I first tried the Zymoclean kit from a free sample and was highly satisfied with the yield and purity of the extracted DNA, as well as the ease of use of the kit. In particular, I had found that competing products often had either low yields or impurities, or in some cases both, whereas the Zymoclean kit was fast, reliable and effective. It was not a difficult decision to place an order once the free sample had run out!"

"We use this product to gel purify after cloning our vectors. It works very well and is cost effective."

"This product makes isolating a size specific DNA band from a gel super easy & reproducible!"

"The product is easy to use, and what I really found beneficial is that the results are always reproducible and clean, the amount of recovered DNA depends on the elution. I always cut the fragment under low UV lamp, as quick as possible, and I recommend to eluate the sample in 15 ul, therefore the eluated volume is around 12 ul. I suggest using warm water (37 C) or 0.1mM Tris/EDTA for the elution, not cold water. What is another positive characteristic of ZymoPure kits, is that the gel recovery and DNA clean up kit components are interchangeable. Time management is good, but the written time is not enough for me to complete many samples."

"Straight forward instructions make this kit easy to use with no prior experience. Results were acceptable and reproducible."

"This gel purification kit has only few steps and its easy to use even for someone without previous molecular biology experience. I have used this kit routinely to isolate both small PCR fragments as well as larger vector backbones. I always get good quality DNA and this has made my cloning so much more efficient. This kit is priced competitively and that makes it even more attractive. Please continue to make more such great products!"

"Ease of use: very easy and quick. The instructions are simple to follow and the protocol takes just a small amount of time. After sales care: good communication with the company, I have never had any problems or a defective kit. Value for money: great value! I get high DNA recovery with my use and this is an invaluable product to have in my lab."

"I used the Zymo DNA recovery kit to extract and purify DNA sequences where I look for mutations causative of human diseases. Protocol is pretty easy to follow and is kind of short, not time consuming. Also, quality of sequences obtained from the extraction with the kit is reliable, reproducible and I get good quality sequence readings. From my experience, the product is good and the price is in accordance with the quality of the service. Besides that, support team and sales people are always able to help either by call, stop by the lab or even at vendor shows, trying to help and offering solutions."

"Product is easy to use. I normally have to follow with a Zymo Clean and Concentrator kit as well which is also easy to use. Product does take long to get to me when the order is placed."

"Easy to use, instructions are very clear, good value for the money."

"The product yields a decent amount of DNA from the electrophoresis gel that is always clean enough for downstream application."

"This kit is super fast and easy to use, and always gives good yields compared to other kits we've tried. I use weekly and would recommend to anyone!"

"I would recommend this product for DNA extraction from gel."

"Compared to kits from many other companies, you can elute your DNA in very small volumes (<10 ul), which is convenient if you want to use the whole amount for further processing like restiction/electrophoresis. The DNA solubilization/binding/elution procedure is fast, simple and straightforward."

"Zymo DNA elution products are very good and the recovery rates were more than or equal to those of Qiagen gel elution kits."

"This product is easy to use, and always produces good results. I recommend this product to collaborators."

"Very straightforward kit that is a major benefit in purifying low-yield PCR reactions or cloning products. Makes cloning in these fragments much easier, since you can maximize the DNA concentration from the gel."

"The kit gives great yield time and time again. The quality of the purification is always immaculate when checked on the nanodrop and using the ratio indicators."

"Best product available for our undergraduate research. It is relatively inexpensive and easy to use."

"This kit is extremely quick and easy to use. The protocol is written well but can very easily be optimized based on your application."

"I ordered the Zymoclean Gel DNA recovery kit because I want to purify large fragments (6, 10, 15 KB) from agarose gel. I was facing a lot of trouble isolating this using other kit so I wanted to give a try with Zymo kit. I went through the protocol and as per suggestion I did all the steps. I started with a total plasmid DNA (30ng) which I digested with a restriction enzyme and I recovered around 10 ng of the DNA fragment of interest which is atleast 3 times better than the other kits I was using for the same purpose earlier. Yeld is better and the purity is also good. The kit meet my expectations."

"High quality of DNA in a very small final volume - perfect for downstream applications."

"The kit is easy to use, fast and performs well. The kit has good value for money."

"With this kit, which is easy to use, you receive a high quality of clean DNA in a short amount of time which you can directly send for sequencing. You can elute DNA in a small volume, which is great for cloning and sequencing, too."

"This DNA recovery kit is the best I know. The DNA is very clean and in a small volume. You will have a good concentation to work with. The DNA is functional for all other applications. You can ligate the DNA easily and do sequence analysis with the material."

"The product is very easy to use and achieve high yields of DNA. The price is very competitive too and I would highly recommend this product."

"Works very nicely and reproducible results. It is very easy to use and provides good yield."

"The Zymo Research Gel DNA Recovery Kit is the best gel recovery kit on the market. The column shape prevents wash buffer from entering the cleaned sample, which directly translates to more successful downstream applications. I refuse to use other kits than this one!"

"Easy to use, never had any problem with the product neither the service of the company."

"Kit is very easy to use, yields a good amount of product, protocol is succinct and clear."

"All gel extraction kits are a little disappointing, but this product is a great value and performs on par to others."

"I obtained good results with the DNA purification columns."

"Easy to use, following directions get proper-sized DNA no problem."

"One of the best kits for DNA gel purification. The quality/price ration is really good. This kit has a great advantage - you can elute your sample in small volume - 6ul. It's great for molecular cloning, when you need to purify PCR product. Final concentration is enough for ligation. Especially, in cases when PCR yield isn't good. Kit is easy to use, has a brief manual, which is a good point too."

"Very easy to use. DNA can be eluted in a very small volume and yield is good enough for downstream applications (cloning, sequencing)."

"The Gel DNA kit is quick and efficient. However, I consistently have extremely low yields, as low as 1/3rd the quantity of product expected."

"Very easy to use, short protocol, good clean recovery."

"Zymo provides an easy-to-use, highly reliable, gel clean-up kit. They have amazing customer service too!"

"Great purification with little loss. Elute volumes are low making it easy to have a high concentration of gel extracted DNA for further use in ligations or PCR."

"I always have limited DNA loss and perfectly clean DNA after using this kit. It is somewhat time consuming, but worth it in the end."