Zetasizer µV by Malvern Panalytical

The dual capability Zetasizer µV is dedicated to the measurement of proteins, and uses dynamic light scattering (DLS) to measure size, and static light scattering (SLS) to measure molecular weight.

The Zetasizer µV is a highly sensitive dual function light scattering detector. It can be used as a RALS detector with any GPC/SEC system, including Viscotek, to give absolute size (by DLS) and absolute molecular weight (by SLS). Alternatively, in cuvette mode, it is suitable for the detection of aggregates and for monitoring aggregate formation using DLS. The Zetasizer µV can be connected to any GPC/SEC system and concentration detector using an 8µl quartz flow cell and standard chromatography tubing.  This allows the measurement of absolute size, absolute molecular weight, aggregation, and conjugation (when used with a second concentration detector).

Zetasizer µV Features:

• Use as a chromatography detector to measure absolute size and absolute molecular weight, aggregation and conjugation by connecting the Zetasizer µV to your GPC/SEC system and concentration detector, using standard chromatography tubing.
• Use in cuvette mode - simply remove the flow cell from the optics unit and insert a cuvette containing the sample.
• Switch in seconds from chromatography detector to cuvette mode.
• Accurate and repeatable results using precise temperature control.
• Sensitivity – 0.1mg/mL 15kDa protein (Lysozyme).
• Only 2µL sample required in cuvette mode to preserve sample.
• 8µL flow cell for chromatography mode ensures minimal band broadening to preserve the resolution of the separation.
• Connect to any Viscotek system for full software integration and truly automated operation, or third party SEC system using OmniSEC software and OmniFACE interface.
• Automated temperature trends for aggregation temperature determination (melting point).