Zero Blunt® PCR Cloning Kit without chemically competent E. coli by Thermo Fisher Scientific Invitrogen
Manufacturer Thermo Fisher Scientific Invitrogen
The Zero Blunt® PCR Cloning Kit offers an easy method for high-efficiency (>=95%) cloning of blunt-end PCR products amplified with proofreading thermostable DNA polymerases. The Zero Blunt® PCR Cloning Kit uses the multipurpose cloning vector pCR®-Blunt and a one-hour ligation step.The vector features:ccdB gene for positive selectionR I sites flanking the PCR product insertion site for excision of insertsChoice of kanamycin or Zeocin™ resistance for flexible antibiotic selectionT7 promoter/prime... Read more
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Zero Blunt® PCR Cloning Kit without chemically competent E. coli
The Zero Blunt® PCR Cloning Kit offers an easy method for high-efficiency (>=95%) cloning of blunt-end PCR products amplified with proofreading thermostable DNA polymerases. The Zero Blunt® PCR Cloning Kit uses the multipurpose cloning vector pCR®-Blunt and a one-hour ligation step.
The vector features:
The vector features:
- ccdB gene for positive selection
- R I sites flanking the PCR product insertion site for excision of inserts
- Choice of kanamycin or Zeocin™ resistance for flexible antibiotic selection
- T7 promoter/primer site for in vitro RNA transcription and sequencing
- M13 forward and reverse primer sites for sequencing or PCR screening
Product Overview
Zero Blunt® PCR Cloning Kit without chemically competent E. coli
Manufacturer Thermo Fisher Scientific Invitrogen
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