ZEISS ELYRA P.1 by ZEISS Research Microscopy Solutions
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ELYRA P.1 takes light microscopy to the limit. By localizing small structures and even single molecules, you are able to achieve resolutions of down to 20 nm laterally and 50 nm axially. Use ELYRA P.1 and photoactivated localization microscopy (PALM) for endogenously-expressed photo-switchable fluorescent proteins.
You are interested in processes that take place near the coverslip. You want to see and measure single molecules in or near the plasma membrane like lipid rafts, receptor clustering or cell-substrate adhesion sites. With 3D-PALM you use photo-switchable proteins and profit from an excellent z capture range.
The patented exclusive PALM technology of ELYRA P.1 takes you into a new world of data quality. Detection with an effective resolution down to 20 nm will show you substructure and patterns where conventional light microscopy will simply show co-localization.
As a single molecule method, PALM is inherently quantitative – every image is a molecular statistics experiment.
Applications:
- Probe the structural organization of a whole cell.
- Investigate arrangement of cellular components and proteins.
- Explore interaction of molecules.
- Reveal the ultrastructure of organelles.
- Probe the ultrastructure of molecular assemblies.
- Map protein localization onto a structural context.
- Track many molecules and retrieve diffusion behavior.
- Study structural changes of slower dynamics.
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