More than 18,000 studies have been published citing the use of the yeast two-hybrid system to investigate protein-protein interactions, and Clontech’s Matchmaker™ Systems have been by far the most popular. Matchmaker™ Gold Systems are the highest performing incarnations of our Matchmaker product line, due to a combination of a new yeast strain (Y2HGold), stringent reporters, easy to use libraries, high-level expression vectors, and SMART™-based library construction kits. In a Matchmaker GAL4-based two-hybrid assay, a bait protein is expressed as a fusion to the GAL4 DNA-binding domain (DNA-BD), while libraries of prey proteins are expressed as fusions to the GAL4 activation domain (AD; Fields & Song, 1989; Chien et al., 1991). When bait and library (prey) fusion proteins interact, the DNA...Read more
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